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KMID : 0368420040470010001
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Journal of Plant Biology
2004 Volume.47 No. 1 p.1 ~ p.1
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Studies on the mobilization of a maize transposable family,Ac/Ds, in pepper using In Vivo transient assay system
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Kim Chul-Min
Je Byoung-Il
Koo Ja-Choon
Piao Hai Long
Park Soon-Ju
Jeon Joo-Mi
Kim Min-Kyoung
Park Sung-Han
Park Jin-Young
Lee Eun-Jin
Chung Woo-Sik
Lee Kon-Ho
Kang Kyu-Young
Lee Sung-Ho
Han Chang-Deok
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Abstract
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A maize transposable family, Ac/Ds, has been successfully utilized as a mutagenizing agent not only in monocot but also in dicot. In order to develop insertional mutagenesis system in pepper, the mobility of Ac/Ds has been examined. In this study, the excision of the elements was monitored via transient assay system with protoplasts. Two different systems were developed and compared; one- and two-elements systems. In a one-element system, Ac alone was introduced into cells. As a two-element system, Ac and Ds were cloned into a single vector and were expressed in protoplasts. Our data showed that both Ac and Ds elements were highly mobile in pepper cells. This is the first report suggesting that Ac/Ds mediated gene tagging system could be successfully operated in pepper.
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KEYWORD
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Ac/Ds, pepper, transient assay
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